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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Invest Dermatol. 2017 May;137(5):e93–e99. doi: 10.1016/j.jid.2016.04.040

Figure 1. Changes in the spatial organization of the keratinocyte nucleus during epidermal development and differentiation.

Figure 1

A – 3D-FISH image of the nucleus of murine basal epidermal keratinocyte showing the positioning of the chromosomes 3 and 15 (arrows). Chromosome territory 3 (CT3, pink/violet) occupy more peripheral positioning in the nucleus, while the chromosome territory 15 (CT15, green) show more central positioning (courtesy of I. Malashchuk).

B - Chromosomes occupy distinct territories, in which distinct chromatin domains are permeated by interchromatin channels connected with a network of larger channels and lacunas separating distinct chromosomes and harboring different nuclear bodies including speckles (see Cremer et al., 2015, for details).

C – 3D-FISH image of the nucleus of murine basal epidermal keratinocyte showing the chromosome territory 3 (CT3, yellow) with EDC locus located at the internal part of the CT3 (red) (courtesy of I. Malashchuk).

D – Scheme illustrating the remodeling of 3D nuclear organization during terminal keratinocyte differentiation in the epidermis (see Gdula et al., 2013, for details).