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Co-elution of p137 and Sec13p by column chromatography. The supernatant of the microsomal fraction was applied sequentially to hydroxyapatite, Sephacryl S300, and MonoQ HR 5/5 columns. The eluted fractions were solubilized and separated on SDS-PAGE (8% acrylamide), then subjected to immunoblot analysis using antibody 212 (to detect p137) and anti-human Sec13 antibody. The elution profiles for both protein bands coincide at each chromatography step.
