Fig. 8.

The vesicular compartment containing p137 colocalizes with transfected Sec13p. Clone 9 cells (top row) or NRK cells (bottom row) were transfected with Sec13p-GFP or Sec13p with a T7 tag, respectively, fixed, then incubated with anti-p137 alone (Clone 9) or together with anti-T7 antibody (NRK). Sec13p was localized to cytoplasmic vesicles in both cell lines; it was also found over the nucleus in some cells. p137 was distributed to small cytoplasmic vesicles, but not over the nucleus. Most of the vesicular labeling for p137 and Sec13p overlapped (merged images). Bars, 10 μm.