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. 2017 Aug 23;16:148. doi: 10.1186/s12934-017-0762-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression and surface display of mouse IL-22 by L. paracasei BL23. a Detection of mouse IL-22 in the supernatant (sup) or cell pellet (cell) of Lp pAFβ100-IL22 (secreted-IL-22) and Lp pAFβ900-IL22 (anchored IL-22) by Western blot. b Surface display of anchored IL-22 (Lp pAFβ900-IL22) by flow cytometry. For both methods, the initial pH of culture MRS medium was either adjusted to pH 8.5 or not adjusted (pH 6.3)