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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Mol Neurosci. 2001 Dec;17(3):285–292. doi: 10.1385/JMN:17:3:285

Fig. 2.

Fig. 2

Effect of the A3 receptor agonist Cl-IB-MECA on the expression of Bcl-2 and Bax. Cells were treated with Cl-IB-MECA (25 μM) for 12 and 24 h. The cells were then harvested and immunoprecipitation of Bcl-2 was performed as described in the Methods or cells were extracted for Western-blot analysis. Following SDS-PAGE, membranes were stained with anti-Bcl-2 antibody or with anti-Bax antibody. The results represent one of three separate experiments, which gave similar results.