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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Biochem Pharmacol. 2001 Mar 15;61(6):657–663. doi: 10.1016/s0006-2952(01)00531-7

Fig. 5.

Fig. 5

(A) Saturation isotherm of [3H]MRS 1754 binding to human A2B receptors expressed in HEK-293 cell membranes, and (B) Scatchard analysis of the same data. [3H]MRS 1754 (0.1 to 20 nM) was incubated with 30 μg of membranes for 60 min at 25°. Total binding (○), specific binding (●), and nonspecific binding (Δ), determined using 100 μM NECA, are shown in (A). Four experiments were carried out; data from one representative experiment are shown.