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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Mol Psychiatry. 2007 Apr 17;13(2):162–172. doi: 10.1038/sj.mp.4001991

Figure 2.

Figure 2

Co-precipitation of MAGI-2 and ERBB4. Cells were transfected with: (1) MAGI-2; (2) EICD (top panels) or ERBB4 (bottom panels); (3) vector; or (4) MAGI-2 and either EICD (top panels) or ERBB4 (bottom panels). Cells were lysed and subjected to immunoprecipitation (IP) with anti-HA antibody (to precipitate HA-tagged MAGI-2), and the amounts of EICD, ERBB4, or cleaved, C-terminal fragment of ERBB4 (CTF), were determined by immunoblotting (IB) with an antibody (C-18) against the C terminal of ERBB4 (left panels). In parallel, an aliquot of cell lysate from transfected cells (10% of the amounts used for the immunopreciptation) was immunoblotted to confirm expression of transfected proteins (right panels).