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. 2001 Jul;12(7):2219–2228. doi: 10.1091/mbc.12.7.2219

Figure 2.

Figure 2

Rabaptin-5 enhances Rabex-5 GEF activity. GDP to GTP nucleotide exchange on Rab5 was measured by filter binding assay in the presence of 5 μM [32P]GTPγs for the indicated times. This was achieved with 1.5 μM Rab5 alone (▴) or with 100 nM Rabex-5 (✦), 100 nM Rabex-5 and 100 nM Rabaptin-5 (●), or 100 nM Rabaptin-5/Rabex-5 complex (▪).