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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Sci Transl Med. 2017 Jun 28;9(396):eaal3653. doi: 10.1126/scitranslmed.aal3653

Figure 1. MERS-CoV antiviral efficacy, toxicity and metabolism of GS-5734 in 2B4 cells.

Figure 1

(A) Mean percent inhibition of MERS-CoV replication by GS-5734. 2B4 cells were infected in triplicate with MERS-CoV nLuc at an MOI of 0.08 in the presence of varying concentrations of GS-5734 for 48hr after which replication was measured through quantitation of MERS-CoV expressed nano luciferase (nLUC). (B) Cytoxicity in 2B4 cells treated similarly to those in A. Viability was measured via CellTiter-Glo. Data for A and B is representative of three independent experiments. (C) Measurement of intracellular nucleotide triphosphate (NTP) in 2B4 cells. In three independent experiments, triplicate wells of cells were treated with 1 μM GS-5734 and harvested over time to measure NTP via LC/MS.