(A) Pharmacokinetics in Ces1c−/− mouse plasma following subcutaneous administration of 25mg/kg GS-5734. Longitudinal plasma samples were taken to measure prodrug GS-5734, intermediate metabolites Ala-Met, and nucleoside by LC/MS. (B) Lung triphosphate in Ces1c−/− mouse lung 4 hr following subcutaneous administration of 50mg/kg GS-5734. Nucleotide monophosphate (Nuc-MP), diphosphate (Nuc-DP), triphosphate (Nuc-TP) and total nucleotide (sum of Nuc-MP, DP and TP) is displayed. (C) Pharmacokinetics of total nucleotide in Ces1c−/− mouse lung following subcutaneous administration of GS-5734 at 25mg/kg twice daily (BID) or 50mg/kg once daily (QD).