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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Cancer Res. 2016 Oct 3;76(22):6657–6668. doi: 10.1158/0008-5472.CAN-16-0568

Figure 1. EWS-FLI1 changes localization upon trabectedin or lurbinectedin treatment.

Figure 1

A) Single-cell imaging of HA-tagged TC32 cells treated with 5 nmol/L trabectedin or lurbinectedin for 6 hours shows EWS-FLI1 (green) localization into the nucleolus (red). DAPI (blue) was used as a nuclear stain. B) Single-cell imaging of HA-tagged TC32 cells treated with ET-745 or etoposide shows a lack of EWS-FLI1 nucleolar localization. C) Single-cell imaging of TC32 cells treated with 5 nmol/L Trabectedin, Lurbinectedin, or ET-745 for 6 hours shows the appearance of γH2AX (green) foci. DAPI (blue) used as a counterstain. Scale bars = 10 μm throughout this figure.