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Serum HAI and neutralization titers after MNP boost. Sera from mice were collected at 3 and 7 weeks post the primary immunization and analyzed for hemagglutination inhibition (HAI) titers against Aichi (A) and PR8 (B) influenza viruses, respectively. (C) Serum neutralization titers against Aichi and PR8 viruses were detected by microneutralization assay following the protocol described in the WHO manual. Data represent mean±SD. *: p<0.05, **: p<0.01, ***: p<0.001.
