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Quantitative PCR (qPCR) amplification curves reveal successful amplification from all synthetic Mars analog soils (perchlorate, alkaline, aeolian, acid, salt, JSC, and basalt) minus one alkaline sample (likely due to pipetting variability) suggest high PureLyse elution purity (1 ng of B. subtilis DNA, 10 ng to 10 pg and NTC standards, n = 3). (A) Raw fluorescence (B) Normalized fluorescence.
