FIG. 4.

Three-dimensional culture of iPS-ECs in microfluidic devices. (A) Schematic illustration of the microfluidic device. The cell suspension in fibrin is loaded into the tissue chamber region, while media are delivered through the top and bottom fluidic lines. (B) Representative images of the vessel network formation by CDH5-iPS-ECs tracked over a period of 14 days. (C) The vessel anastomoses to the top fluidic line as indicated by the continuous CDH5-iPS-EC lining around the opening of the tissue chamber. (D) The microvessels demonstrate a patent lumen as confirmed by confocal microscopy. The dashed box region: (E) the iPS-ECs deposit laminin as a part of the basement membrane. (F) The vessel network effectively retains 70 kDa dextran introduced through the top fluidic line. (G) One micrometer bead (red) is captured flowing through the vessel. Scale bar: 200 μm (B, F), 50 μm (C), 100 μm (D), 25 μm (E, G). Color images available online at www.liebertpub.com/tec