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. 2017 Aug 22;16:140. doi: 10.1186/s12943-017-0703-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — F3.EGFRviii cells as cancerous neural stem cells. a Accelerated proliferation of F3.EGFRviii cells relative to F3 cells was determined by cell counting at the indicated times. b Representative images from a clonogenic assay with F3 and F3.EGFRviii cells (top panel). The graphs show the mean number and size of colonies. c Images of colony formation in soft agar (left panel), and the colony number and mean colony size were presented (right panel). d-f The expression of neural stem cell and brain tumor markers in F3, F3.EGFRviii, and U87 cells was determined by real-time PCR (n = 3)