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. 2017 Aug 22;12(8):e0182836. doi: 10.1371/journal.pone.0182836

Table 1. Details of primers and their amplification conditions used in the study.

Region Primer Sequence (5´-3´) Thermocycling conditions Reference
ITS ITS5a 5'-CCTTATCATTTAGAGGAAGGAG-3' 94°C 5 min; [30 cycles: 94°C 1 min; 50°C 1 min; 72°C 1.5 min]; 72°C 7 min [23]
ITS4 5'-TCCTCCGCTTATTGATATGC-3'
rbcL rbcL1F 5'-ATGTCACCACAAACAGAAAC-3‘ 95°C 2 min; [35 cycles: 94°C 1 min; 55°C 30 s; 72°C 1 min]; 72°C 7 min [23]
rbcL724R 5'-TCGCATGTACCTGCAGTAGC-3‘
matK matK 390F 5'-CGATCTATTCATTCAATATTTC-3’ 95°C 2 min; [30 cycles: 94°C 1 min; 48°C 30 s; 72°C 1 min]; 72°C 7 min [23]
matK 1326R 5'-TCTAGCACACGAAAGTCGAAGT-3'
psbA-trnH fwdPA 5'-GTTATGCATGAACGTAATGCTC-3 94°C 5 min; [35 cycles: 94°C 1 min; 55°C 30 s; 72°C 1.5 min]; 72°C 7 min [39]
revTH 5'-CGCGCATGGTGGATTCACAATCC-3'
psbA 5'-GTTATGCATGAACGTAATGCT-3' 94°C 5 min; [35 cycles: 94°C 1 min; 55°C 30 s; 72°C 1.5 min]; 72°C 7 min [23]
trnH 5'-CGCGCATGGTGGATTCACAATCC-3'