Table 3. Identification success rates based on analysis of the ‘best match’, ‘best close match’ and ‘all species barcodes’ function of TaxonDNA software for each DNA barcoding marker and its combinations.
| Barcode locus/loci (No. of sequences) |
Best close match* | All species barcode* | ||||
|---|---|---|---|---|---|---|
| Correct (%) | Ambiguous (%) | Incorrect (%) | Correct (%) | Ambiguous (%) | Incorrect (%) | |
| rbcL (90) | 34.44 | 64.44 | 1.11 | 27.77 | 70.0 | 2.22 |
| matK (80) | 75.24 | 6.62 | 18.13 | 35.0 | 56.25 | 5.0 |
| ITS (51) | 76.47 | 7.84 | 15.68 | 35.29 | 50.98 | 13.72 |
| rbcL+matK (62) | 55.63 | 34.23 | 10.13 | 30.64 | 66.12 | 1.61 |
| rbcL+ITS (45) | 82.22 | 4.44 | 13.33 | 40.0 | 48.88 | 11.11 |
| matK+ITS (36) | 94.44 | 0.0 | 5.55 | 33.33 | 66.66 | 0.0 |
| rbcL+matK+ITS (32) | 93.75 | 0.0 | 6.25 | 37.5 | 62.5 | 0.0 |
* Identification analysis was performed at 3% threshold. Highest values among the single and combination of barcodes for each function of the program are given in bold. The preferred barcoding option for identification of the Terminalia is highlighted in grey.