Table 4. The frequencies of correct identification (CI) for all samples based on sequence similarity method, pair-wise genetic distance method and tree-based analysis.
Barcode locus/loci (No. of Sequences) | CI Best close match (%) | DNA Barcoding gap (%) | Tree based (CI individuals) † | ||
---|---|---|---|---|---|
NJ | ML | MP | |||
rbcL (90) | 34.44% | 33.0% | 36 | 46 | 31 |
matK (80) | 75.24% | 56.6% | 43 | 47 | 39 |
ITS (51) | 76.47% | 69.2% | 39 | 33 | 39 |
rbcL+matK (62) | 55.63% | 26.8% | 50 | 44 | 28 |
rbcL+ITS (45) | 82.22% | 31.5% | 37 | 31 | 33 |
matK+ITS (36) | 94.44% | 87.2% | 28 | 26 | 26 |
rbcL+matK+ITS (32) | 93.75% | 55.65% | 30 | 27 | 27 |
†Based on the proportion of monophyletic species with 60% bootstrap.