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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Radiat Res. 2017 Feb 22;187(3):367–381. doi: 10.1667/RR14623.1

Fig. 3.

Fig. 3

MnTnTE-2-PyP and NOX4−/− prevents contraction of collagen by primary prostate fibroblasts. Panel A: Mouse primary prostate fibroblasts were grown in rat collagen discs. Wild-type fibroblasts grown in either PBS or 30 μM MnTE-2-PyP were either 2 Gy X-ray irradiated or treated with 5 ng/ml TGF-β1. NOX4−/− fibroblasts were either 2 Gy X-ray irradiated or treated with 5 ng/ml TGF-β1. Panel B: Collagen disc area measurements were performed with ImageJ. Collagen discs were significantly contracted in wild-type 2 Gy irradiated fibroblasts (P=0.02) and TGF-β1 (P=0.0001). There was no significant change in collagen disc contraction in cells treated with MnTE-2-PyP or cells lacking NOX4. All data are representative of the mean and standard deviation and were obtained from three independent experiments. P values and significance were determined using one-way ANOVA followed by post hoc Tukey's test for multiple comparisons.