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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Radiat Res. 2017 Feb 22;187(3):367–381. doi: 10.1667/RR14623.1

Fig. 5.

Fig. 5

MnTnTE-2-PyP and NOX4−/− prevents radiation-induced senescence in primary prostate fibroblasts. Mouse prostate fibroblasts maintained in media with PBS or 30 μM MnTE-2-PyP (for wild-type mice prostate fibroblasts) were either sham or 2 Gy X-ray irradiated, and 48 h later, cells were stained for SA-β-gal expression. Cells were imaged with an inverted brightfield microscope. SA-β-gal expression was significantly increased (P = 0.005) in 2 Gy irradiated control cells compared to the nonirradiated population. In wild-type cells, grown in MnTE-2-PyP, and the NOX4−/− cells showed no significant increase in SA-β-gal expression after irradiation. All data are represented by the mean ± standard deviation and were obtained from three independent experiments. The differences of the percentage mean of senescent cells were analyzed for significance using one-way ANOVA followed by post hoc Tukey's test for multiple comparisons.