Table 1.

Identification of the rpoB mutation for rifampicin resistant M. tuberculosis strains using GeneXpert, Hains Genotype MTBDRplus and Sequencing (Bold: novel mutations in the RRDR)

Altered codons	Nucleotide change(s)	Amino acid change(s)	No. and rate of strain with mutations
507	GGC-AGC	G507S	1
508	ACC-GCC,CCC,AAC	T508A, T508N	1
511	CTG-CCG, GTG	L511P, L511V	3
512	AGC-AAC	S512N	2
513	CAA-deletion	Q513del	1
514	TTC-deletion	F514del	2
515	ATG-deletion	M515del	3
516	GAC-deletion, GGC, TAC	D516del, D516G, D516Y	8
518	AAC-deletion, CAC	N518del, N518H	3
519	AAC-deletion	N519del	1
520	CCG-CCC, ACG	P520P, P520T	2
524	TTG-TTA	L524L	1
526	CAC- deletion, GAC, CTC	H526del, H526D, H526L	4
528	CGC-CAC	R528H	2
529	CGA-CAA	R529Q	1
531	TCG-TTG, TTC, TGG	S531L, S531W, S531F	25
533	CTG-CCG	L533P	1
Mutations outside the rifampicin resistant determing region (81bp)
500	GCC-GAC, ACC	A500D, A500T	1
502	ATC-ACC	I502T	2
503	AAG-AAC,AAA	K503N, K503K	10
504	GAG-AAG	E504K	28
505	TTC-TTG, TTT	F505L. F505F	2
506	TTC-TTG	F506L	1
490	CAG-GAA,TAG,CAA,TAT	Q490E, Q490Y, Q490Q, Q490STOP	17
562	GAA-GAT,GCA,CAA,GGT	E562D, E562A, E562Q, E562G	9
564	CCT-CCA, delT,TCG	P564P. P564S, P564del	3
553	TCG-deletion, TCA	S553del, S553S	15
572	ATC-CCG, TTC	I572P, I572F	3
471	ATG-GTG,CTG,delAG,A-G, CAG, GAG	M471V, M471L, M471del, M471E	35
559	TGC-delT, ATT, GTC, TCC	C559del, C559C, C559Y	26
557	CGG-CAG, CAA. GGG	R557Q, R557G	7
555	TAC-CAC, TCC, TTC, TAA, TCA, ACA	Y555H, Y555S, Y555F, Y555T, Y555S, Y555stop	34
543	GCC-ACG, TAC, AC-.-CC	A543T, A543Y, A543del	12
No mutation			6
547	GTC-GTT,GTA, -GT, -TT	V547V, V547del	18
567	CCC-ACC, -CC	P567T, P567del	10
570	GGT-GAT	G570D	10