Fig. 1.

Preparation of Xenopus egg extract. Flow chart indicating individual steps to prepare Xenopus egg extract. Frog eggs are covered with a sticky jelly coat that must be removed prior to extract preparation. Harvested eggs are washed and de-jellied with cysteine before performing a packing spin to remove residual buffer. The crushing spin separates the egg extract into a cytoplasmic, yolk, and lipid layers. The cytoplasmic layer is isolated and used to perform β-catenin degradation assays