Skip to main content
. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Methods Mol Biol. 2016;1481:101–109. doi: 10.1007/978-1-4939-6393-5_11

Fig. 3.

Fig. 3

Reconstitution of β-catenin-luciferase degradation in Xenopus egg extracts. In vitro-translated β-catenin-luciferase fusion was incubated in Xenopus egg extracts in the presence of buffer, Axin (50 nM), or lithium chloride (20 mM; inhibitor of GSK3). At the indicated times, an aliquot was removed and luciferase activity measured. Background signal observed in the β-catenin-luciferase degradation assay is due to free luciferase protein, which degrades slowly