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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2015 Aug 20;54(41):11983–11988. doi: 10.1002/anie.201504902

Figure 3. NanoScript-GFP Effectively Silences GFP Expression.

Figure 3

(a) Schematic representation of NanoScript-GFP downregulating GFP expression (i.e. reduction in green fluorescence from the cell) in GFP-labeled rat NSCs. (b) Fluorescence images of GFP-labeled rNSCs 4 days post transfection showing a maximal decrease in GFP expression (green) when NanoScript-GFP is transfected (scale bar = 20 μm). A 1 nM concentration of MCNPs was applied during the transfection. (c) Quantification of GFP expression in the fluorescence images corroborates the trend in the images and reveals that NanoScript-GFP has the highest GFP knockdown as compared to the controls. Quantification of GFP knockdown is an average from 6 images and standard error is from three independent trials.