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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2015 Aug 20;54(41):11983–11988. doi: 10.1002/anie.201504902

Figure 4. NanoScript-Sox9 Represses Sox9 to Induce Functional Neuronal Differentiation.

Figure 4

(a) Schematic representation of Sox9 repression in human NSCs by NanoScript-Sox9 induces enhanced neuronal differentiation. (b) Fluorescence images of hNSC stained with Tuj1 5 days post-transfection shows greater Tuj1 expression (red) when NanoScript-Sox9 is transfected. (Scale bar = 20 μm). (c) Gene expression analysis using qPCR in hNSCs reveals that repression of Sox9 correlates with an upregualtion of Tuj1. (Percent down-regulation of Sox9 and fold up-regulation of Tuj1 was calculated by normalizing to the housekeep gene, GAPDH, from the control) Standard error is from three independent trials (* = P < 0.05). (d) Spontaneous calcium fluctuations via Fluo4 fluorescence (orange/yellow color) for an active neuron (white circle) during 18 seconds of imaging (scale bar = 20 μm). (e) Traces for the normalized fluorescence change (ΔF/F0) representing spontaneous calcium ion influx for an active neuron (red line) and an inactive neuron (black line). Decreasing trend of the fluorescence is due to mild photobleaching.