Figure 7.

Interconnection between Feeding and Circadian Rhythms in Regulating NAD⁺ Synthesis and Circadian Clock Disruption Effects on Lysine Succinylation and Malonylation

(A) Rhythmic feeding effect (blue) versus the circadian clock (red) on key components implicated in acetyl-CoA-dependent acetylation and the regulation NAD⁺ synthesis during the day (fasting phase) and night (feeding phase).

(B and C) Western blot analysis of global lysine succinylation (B) and malonylation (C) performed on total protein extract from Cry1/2 DKO, Bmal1 KO, and their corresponding WT control mice (left). Naphtol blue black staining of the membrane was used as a loading control (center) and served as a reference for normalization of the quantified values displayed at the right. Average acetylation levels were compared between clock-disrupted mice and their controls using Student’s t test (n = 4). Error bars represent min to max.