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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Nat Protoc. 2016 Nov 10;11(12):2432–2453. doi: 10.1038/nprot.2016.142

TABLE 1.

Sample size calculation.

Drug effect
(% of positive control)		 Normalized scaling factor Minimum sample no Type I (α) error Type II (β) error
P Zα P Zβ
Raw signal
100% 1.000 15.14 0.05 1.96 0.2 (80% power) 0.84
75% 1.249 17.25 0.05 1.96 0.2 (80% power) 0.84
50% 1.663 21.89 0.05 1.96 0.2 (80% power) 0.84
25% 2.489 39.10 0.05 1.96 0.2 (80% power) 0.84
100% 1.000 25.03 0.05 1.96 0.05 (95% power) 1.64
75% 1.249 28.52 0.05 1.96 0.05 (95% power) 1.64
50% 1.663 36.18 0.05 1.96 0.05 (95% power) 1.64
25% 2.489 64.64 0.05 1.96 0.05 (95% power) 1.64
Log2 signal
100% 1.000   7.05 0.05 1.96 0.05 (95% power) 1.64
75% 1.249   9.41 0.05 1.96 0.05 (95% power) 1.64
50% 1.663 14.73 0.05 1.96 0.05 (95% power) 1.64
25% 2.489 35.19 0.05 1.96 0.05 (95% power) 1.64
100% 1.000 10.00 0.05 1.96 0.01 (99% power) 2.33
75% 1.249 13.36 0.05 1.96 0.01 (99% power) 2.33
50% 1.663 20.91 0.05 1.96 0.01 (99% power) 2.33
25% 2.489 49.97 0.05 1.96 0.01 (99% power) 2.33