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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Immunity. 2017 Aug 15;47(2):310–322.e7. doi: 10.1016/j.immuni.2017.07.013

Figure 4. vic1 is H2-Ob.

Figure 4

Production of anti-virus antibodies by infected B6.H2-Oa−/− (A) and B6.H2-Ob−/− mice (B). Mice were infected with MMTV at 6–8 weeks of age, and at three months (H2-Oa−/− mice) and two months (H2-Ob−/−) post infection their sera were screened for the presence of anti-virus IgG2c Abs by ELISA (left graphs) and for neutralization of MMTV infection in BALB/cJ mice (right graphs). B6 (H2-Oa+/+ and H2-Ob+/+) and B6vic1i/i mice were used as negative and positive controls, respectively. Means are derived from combined data from more than 3 independent experiments. Sera that showed reactivity above the background in ELISA were used for pre-incubation with MMTV virions before injection into susceptible BALB/cJ mice. Neutralization (%) was calculated as described in Methods. n, number of mice (left graphs) or number of sera used (right graphs). Significance was calculated using an unpaired t test. See also Figures S1 and S2.