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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Biochemistry. 2017 Feb 23;56(9):1324–1336. doi: 10.1021/acs.biochem.6b01163

Figure 4. RTX751 block II chimera loses affinity for M1H5.

Figure 4

Several RTX751 chimeras were generated, in which regions of low homology were grafted from B. parapertussis into the B. pertussis RTX751 gene. A, Expression and purification of the three chimeras. Shown is a size exclusion chromatogram on an S200 column. Inset, SDS-PAGE gel with molecular weight markers indicated. B, Binding interactions between the chimeras and antibody M1H5, as measured by ELISA. The chimeras were used to coat the plate, followed by blocking and serial dilution of the antibody. Bound antibody was detected with anti-human-Fc-HRP. C, Binding between the chimeras and M2B10 antibody, evaluated as in panel B.