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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Biochemistry. 2017 Feb 23;56(9):1324–1336. doi: 10.1021/acs.biochem.6b01163

Figure 7. Antibody-binding populations isolated by flow cytometry.

Figure 7

The RTX751 library was induced and stained with antibodies 1H6 and M2B10 or M1H5, followed by sorting on a FACS Aria cell sorter. DNA sequencing revealed that the P4 population, with severely reduced antibody binding, was dominated by clones with internal deletions. The P5 population, with moderately reduced antibody binding, contained full length RTX751 with random mutations.