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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Biochemistry. 2017 Feb 23;56(9):1324–1336. doi: 10.1021/acs.biochem.6b01163

Table 1.

Antibody-RTX751 binding affinities and kinetics

KinExA Reichert SPR
Kd (nM) Chi2 (R2) On-rate (sec−1M−1) Off-rate (sec−1) Kd (nM)
M1H5 1.63 ± 1.21 0.001 (0.995) 2.36×105 3.09×10−4 1.31
M2B10 1.25 ± 0.68 0.00043 (0.998) 1.74×105 1.53×10−3 8.78*
*

Poor data fitting due to incomplete regeneration between cycles.