Table 1.
Primers used for real-time PCR (16)
| Region | Primer Name | Sequence | Amplicon size (bp) |
|---|---|---|---|
| EP1 | EP1-F | 5’-GCTGGACCAGAAAGTGTTGAG-3’ | 149 |
| EP1-R | 5’-GTGTGCTGCTTTGCAATGTG-3’ | ||
| EP4 | EP4-F | 5’-CTGTTGCATGAGAGCAGAGG-3’ | 95 |
| EP4-R | 5’-CGTCCCCCTCGCTACTATCT-3’ | ||
| EP5 | EP5-F | 5’-TGCAGGATATTTGGCAAGGT-3’ | 127 |
| EP5-R | 5’-CTGTGCCGGTAGAAATGGTT-3’ | ||
| EP6 | EP6-F | 5’-TGAATCAGTTCACCGACAGC-3’ | 104 |
| EP6-R | 5’-GAAACAACCTGGCCATTCTC-3’ | ||
| EP7 | EP7-F | 5’-CCGTTATATGGATGCCTTGG-3’ | 127 |
| EP7-R | 5’-AAACTGTTGGGCTGAACTGC-3’ | ||
| EP9 | EP9-F | 5’-GACCCAGACGATACCTGGAA-3’ | 110 |
| EP9-R | 5’-GCTGAACAAAACTCGGCTTC-3’ | ||
| EP12 | EP12-F | 5’-ATTCTCCACAGGGCAATGAG-3’ | 128 |
| EP12-R | 5’-TTATGTGGCCTTTCCTCCTG-3’ | ||
| HYP113c | HYP113 c-F | 5’-CAGGAAAGTGAAGGGAGCTG-3’ | 79 |
| HYP113 c-R | 5’-CAAAACCCAATGGTCAATCC-3’ | ||
| U122d | U122 d-F | 5’-AAGGTGCCCAATTCAAGGTA-3’ | 104 |
| U122 d-R | 5’-CTTCCCCACCAGTCTTGAAA-3’ |