Fig 3. Effect of L-arginine supplementation on relative kidney expression of inflammatory cytokines; interleukin 6 (IL6), interleukin 1β (IL1β) and tumor necrosis factor α (TNFα) mRNA (2^{-Δ ΔCT}) and on on interstitial infiltration of macrophages and lymphocytes at days 5, 10 and 20 in CTL, AA and AA+L-Arg mice.

Real-time quantitative PCR for interleukin 6 (IL6) (A), interleukin 1β (IL1β) (B) and tumor necrosis factor α (TNFα) (C) mRNA expression was performed on kidney tissue from CTL, AA and AA+L-Arg mice normalized against 18S. Macrophage (D) and lymphocyte (E) accumulation within the interstitium of kidney from CTL, AA and AA+L-Arg mice at days 5, 10 and 20. Statistical analysis was performed using two-way ANOVA followed by Holm-Sidak test. Data are presented as means ± SEM; n = 8 in each group. *P ≤ 0,05 vs CTL mice, **P ≤ 0,01 vs CTL mice, ***P ≤ 0,001 vs CTL mice, ^#P ≤ 0,05 vs AA mice, ^##P ≤ 0,01 vs AA mice and ^###P ≤ 0,001 vs AA mice.