Table 1. Sequences of the primers used for qRT-PCR.
Gene | Primer Sequences (5'-3') | |
---|---|---|
eNOS | F | AACCATTCTGTATGGCTCTGAGAC |
R | CTCTAGGGACACCACATCATACTC | |
iNOS | F | CAGCTGGGCTGTACAAACCTT |
R | ATGTGATGTTTGCTTCGGACA | |
nNOS | F | ACCCTGTGCGAGATCTTCAA |
R | TGGTGACCACCAAGACCAG | |
NGAL | F | ATGTCACCTCCATCCTGGTC |
R | CCTGTGCATATTTCCCAGAGT | |
IL-1β | F | AGTTGACGGACCCCAAAAG |
R | AGCTGGATGCTCTCATCAGG | |
IL-6 | F | GCTACCAAACTGGATATAATCAGGA |
R | CCAGGTAGCTATGGTACTCCAGAA | |
TNFα | F | TACTGAACTTCGGGGTGATTGGTCC |
R | CAGCCTTGTCCCTTGAAGAGAACC | |
Col I | F | GCAGGTTCACCTACTCTGTCCT |
R | CTTGCCCCATTCATTTGTCT | |
Col III | F | TCCCCTGGAATCTGTGAATC |
R | TGAGTCGAATTGGGGAGAAT | |
CTGF | F | TGACCTGGAGGAAAACATTAAGA |
R | AGCCCTGTATGTCTTCACACTG | |
Periostin | F | TCGTGGAACCAAAAATTAAAGTC |
R | CTTCGTCATTGCAGGTCCTT | |
TGFβ | F | TGGAGCAACATGTGGAACTC |
R | GTCAGCAGCCGGTTACCA | |
Smad3 | F | GCCACTGTCTGCAAGATCC |
R | AGCTAGGAGGGCAGCAAAT | |
18S | F | CGCCGCTAGAGGTGAAATTCT |
R | CGAACCTCCGACTTTCGTTCT |