Fig. 1.

DBT induces the neurotrophic factor expressions in cultured SH-SY5Y cells. Cultured SH-SY5Y cells were pre-treated with fresh medium/U0126 (10 µM) for 3 h before application of this ancient herbal formula (0.5 mg/mL) for 2 days. The cell lysates were collected to determine the protein expressions by using specific antibody (upper panel). FSK (10 µM) acted as a positive control. Loading control was set as GAPDH. Protein expression level was calculated by a densitometer (lower panel). Values were expressed as the fold of increase as compared to untreated culture. Data were expressed as Mean ± SEM, where n = 4. **p < 0.01; ***p < 0.001 compared with corresponding control group without U0126. ^^p < 0.01; ^^^p < 0.001 compared with corresponding FSK or DBT group without U0126, respectively