Fig. 2.

DBT stimulates transcriptional activities of NGF, BDNF and GDNF in cultured SH-SY5Y cells. Cultured cells, transfected with pBDNF-Luc or pGDNF-Luc or pNGF-Luc, were subsequently treated with a FSK (1–30 µM) or b DBT decoction (0.125, 0.25, 0.5 mg/mL) for 48 h. Cells were collected to determine the luciferase activity. The treatment of U0126 (10 µM) was 3 h before DBT application. Promoter-driven luciferase was expressed as the ratio to the negative control. All values were revealed as Mean ± SEM, where n = 5. **p < 0.01; ***p < 0.001 compared with control