Fig. 3.

DBT induces the phosphorylation of Erk1/2 and CREB. Cultured SH-SY5Y cells were serum-starved for 3 h before the application of DBT (0.5 mg/mL) for different duration. a Total Erk1/2 (T-Erk1/2) and phosphorylated Erk1/2 (P-Erk1/2) and b total CREB (T-CREB) and phosphorylated CREB (P-CREB) were analyzed by specific antibodies (left panel). TPA (100 nM) and FSK (10 µM) played as positive control for the activation of Erk1/2 and CREB, respectively. The band density was measured by densitometer (right panel), and the phosphorylation values were expressed as the ratio to the basal reading where the time zero equaled to 1, values were expressed as Mean ± SEM, where n = 4