Skip to main content
. 2017 Aug 23;12(8):e0183694. doi: 10.1371/journal.pone.0183694

Table 1. Primers used for real-time RTPCR for mRNA expression analysis of UPR pathway genes.

Primer Name Target Gene Primer Type Primer Sequence
XBP1 F1 XBP-1 Forward 5'- cacctgagcccggaggag-3'
XBP1 R1 XBP-1 Reverse 5'- gagttcattaatggcttccagc-3'
RFP F2 RFP Forward 5′-ccctcgtcagggaatcttgaagggcg-3′
GFP R2 GFP Reverse 5′-ggcgcctttggtgctcttcatcttgttgg-3′
ATF4 F1 ATF4 Forward 5′-attctccgggacagactgga-3′
ATF4 R1 ATF4 Reverse 5′-tggccaattgggttcactgt-3′
EDEM1 F1 EDEM1 Forward 5′-acgtcttcggaattgcctt-3′
EDEM1 R1 EDEM1 Reverse 5′-gtcgatctggcgcatgtaga-3′
HERPUD1 F1 HERPUD1 Forward 5'-ccacttgagccgagtctacc-3'
HERPUD1 R1 HERPUD1 Reverse 5'-cagcaccctttgttttggct-3'
CHOP F1 CHOP Forward 5'-cttcgttatccccggctctc-3′
CHOP R1 CHOP Reverse 5′-gactcagctgccatgtctgt-3′
DERLIN1 F1 DERLIN 1 Forward 5′-catcggggactggttcagga-3′
DERLIN1 R1 DERLIN 1 Reverse 5′-tggaagcgatagaggaaggc-3′
CHO-GAPDH F1 GAPDH Forward 5′-tgcccagaacatcatccctg-3′
CHO GAPDH R1 GAPDH Reverse 5′-caggcgacatgtcagatcca-3′