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. 2017 Aug 22;17:142. doi: 10.1186/s12870-017-1078-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Gel and qRT-PCR analysis of GaMYB85 transgenic plants a The confirmation of GaMYB85 gene CDS (771 bp) integration in T₀ generated overexpressed Arabidopsis plants. The DNA of WT (Col-0) was used as negative control (lane 1) and 35S:GaMYB85 DNA was used in (lanes 1–10, 11–16). M; DNA marker III, 1 kb. b Relative expression of GaMYB85 gene in T₂ transgenic lines by qRT-PCR having segregating ratio of 3:1 on the selective medium, three cDNA preparations were used and error bars represented with SD value. AtUBQ10 gene (Accession no: AT4G05320) was used as an internal standard in qRT-PCR