Fig. 5.

Plants overexpressing GaMYB85 perform well in response to mannitol stress a 35S:GaMYB85 and WT seed germination performance on MS supplemented with 0, 100, 200, and 300 mM mannitol. Germination rates were scored on the 10th day. b A plot of the germination rates scored on the 10th day for 35S:GaMYB85 and WT seeds germinated on the different mannitol media. Data are the means ± SE for three replicates of 50 seeds each (*P < 0.05). c Fresh weights of seedlings grown on 0–300 mM mannitol MS medium scored on the 10th day. Each treatment with 10 seedlings was performed in triplicate. Data are the means ± SE (*P < 0.05). d Comparison of root elongation of 5-day-old seedlings of 35S:GaMYB85 and WT plants transferred to 0, 100, 200, and 300 mM mannitol MS for 6 days. Values represent the data from three independent growth assays. Scale bar: 1 cm. e Quantitative comparison of root elongation of 35S:GaMYB85 and WT seedlings on MS supplemented with 0–300 mM mannitol (30 seedlings each). Data are the means ± SE (n = 3). P < 0.05 determine by the t-test