Fig. 1.

qRT-PCR depicting relative EMX2 mRNA expression in a primary stage III colorectal cancer samples and adjacent normal tissue. EMX2 levels in b liver metastases from CRC were further down-regulated compared to primary tumors. Bars indicate mean ± SEM (* P < 0.05, n = 31; ** P < 0.01, n = 31 primary tumors; 29 colorectal liver metastases). c Immunohistochemical staining, revealing EMX2 protein expression (arrowheads) in normal mucosa, but not in corresponding primary tumors or in liver metastases of each 2 representative patients. d Western Blot revealing EMX2 expression in primary tumor and normal mucosa from 2 representative patients