Table 3.

Effect of autoclaving on the physicochemical attributes of CIP-NLCs and PEG-CIP-NLCs (pre and post sterilization).

Formulations		Particle Size (nm)		Polydispersity index (PDI)		Assay		pH		Zeta potential (mV)
		Sterilization Stage		Sterilization Stage		Sterilization Stage		Sterilization Stage		Sterilization Stage
		Pre	Post	Pre	Post	Pre	Post	Pre	Post	Pre	Post
DSPE-CIP-NLCs	CIP-NLCs	190±15	230±7.5	0.26±0.03	0.35±0.05	90.9±4.5	87.1±2.1	5	3.6	−12.2±1.08	−14.6±1.95
PEG(1K)-CIP-NLCs	PEGylated CIP-NLCs	165±12	169±8	0.29±0.01	0.29±0.02	90.2±2.8	89.1±2.6	5	4.76	−1.0±0.02	−1.12±0.01
PEG(2K)-CIP-NLCs		180.6±13	192±8	0.31±0.01	0.32±0.01	90.1±3.1	88.4±3.6	5	4.8	−1.8±0.08	−1.9±0.06
PEG(5K)-CIP-NLCs		217±18	231±9.1	0.37±0.03	0.42±0.02	92.6±1.8	91.9±2.9	5	4.4	−2.6±0.06	−3.4±0.04
DMPE(2K)-CIP-NLCs		176±4.8	191±7.6	0.28±0.02	0.31±0.04	91.4±1.1	89.4±0.67	5	4.58	−2.3±0.03	−2.9±0.053
DPPE(2K)-CIP-NLCs		184±3.6	189±8.5	0.27±0.03	0.3±0.02	93.2±0.9	91.8±1.2	5	4.82	−2.1±0.06	−3.3±0.04