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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Nature. 2016 Jun 15;534(7608):553–557. doi: 10.1038/nature18014

Figure 1. Increased H3R17 dimethylation by CARM1 is critical for proper autophagy.

Figure 1

a, b, Immunoblot analysis of various histone marks and CARM1 in response to glucose starvation (Glc starv.). c, Wild-type (WT), Carm1 knockout (KO) or knock-in (KI) MEFs were subject to immunoblot analysis. The LC3-II/LC3-I ratio is indicated. d, Representative confocal images of GFP–LC3 puncta formation. Graph shows quantification of LC3-positive punctate cells (right). Nuclei counterstained with DAPI. Scale bar, 10 µm. e, Representative TEM images. Scale bar, 2 µm. High magnification of boxed areas is shown on the right. Scale bar, 0.5 µm. Autophagosomes (blue arrows), autolysosomes (red arrows) and multilamellar body (yellow arrow). f, Representative confocal images of GFP–LC3 puncta formation. Ellagic acid (100 µM). Scale bar, 10 µm. Bars, mean ± s.e.m.; n = 5, with over 100 cells; **P < 0.01 (one-tailed t-test) (d, f).