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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Nature. 2016 Jun 15;534(7608):553–557. doi: 10.1038/nature18014

Figure 4. CARM1 exerts a transcriptional co-activator function on autophagy-related and lysosomal genes through TFEB.

Figure 4

a, Binding between CARM1 and TFEB. b, Representative confocal images. Scale bar, 10 µm. c, 2× CLEAR (TFEB RE)-luciferase reporter assays. Bars, mean ± s.e.m.; n = 3. **P < 0.01 (one-tailed t-test). d–f, ChIP assays on TFEB-dependent, CARM1-dependent (d, e) or CARM1-independent (f) promoters after knockdown of TFEB. Bars, mean ± s.e.m.; n = 3. g, Wild-type and Carm1 knockout MEFs transfected with Flag–TFEB were subject to immunoblot analysis. h, Liver tissues from fed or fasted mice treated with vehicle or ellagic acid were subjected to immunoblot analysis (n = 3 per group). i, Expression of autophagy-related genes and lysosomal genes in wild-type mouse livers. Bars, mean ± s.e.m.; n = 3 per group. *P < 0.05, **P < 0.01 (two-tailed t-test).