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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Nature. 2016 Jun 15;534(7608):553–557. doi: 10.1038/nature18014

Extended Data Figure 3. CARM1 is degraded by SKP2-containing SCF E3 ligase in the nucleus.

Extended Data Figure 3

a, Wild-type CARM1 and ubiquitination-defective mutant K471R were analysed for their expression in MEFs after MG132 treatment. b, Interaction between CARM1 and CUL proteins was analysed. c, Lysates were analysed by immunoblot. d, Left, HepG2 cells infected with two different SKP2 shRNAs were subject to cycloheximide (CHX) experiment. Right, protein half-life of CARM1 was quantitatively defined (right). e, Left, CHX experiment in HepG2 expressing wild-type SKP2 or ΔF mutant. Right, protein half-life of CARM1 was quantitatively defined. Data are mean ± s.e.m.; n = 3. **P < 0.01 (one-tailed t-test) (d, e).