Table 1. 31 selected RBP10 targets.
These genes encode mRNAs that were bound to RBP10, and were significantly more abundant in procyclic than in bloodstream-form fractions for either total RNA or polysomal RNA (PC/BS; the results for polysomal RNA are shown). Some of these are already elevated in stumpy forms (I. Roditi, Universität Bern, personal communication). The polysomal mRNAs were also at least 1.5 x and significantly (Padj <0.05) increased after rbp10 RNAi in bloodstream forms (BS RNAi). The effect on polysomal RNA for expression of RBP10 in procyclic forms (PC+RBP10) is also shown. Apart from two genes which were affected by RBP10 expression in procyclic forms, we selected genes with annotated functions. For details see S2 Table sheet 2.
| Gene ID | Annotation | BS RNAi | PC+RBP10 | PC/BS | UAU6 |
|---|---|---|---|---|---|
| Membrane | |||||
| Tb927.10.10250 | EP2 procyclin (EP2) | 5.9 | n/a | 199.5 | 3 |
| Tb927.7.6850 | trans-sialidase (TbTS) | 2.4 | 0.51 | 4.0 | 4 |
| Tb927.8.7340 | trans-sialidase | 1.9 | 0.34 | 5.9 | 3 |
| Tb927.4.3500 | Amastin-like protein | 2 | n/a | 10.4 | 1 |
| Mitochondrion | |||||
| Tb927.11.5050 | fumarate hydratase | 1.5 | n/a | 2.0 | 1 |
| Tb927.9.5900 | glutamate dehydrogenase | 1.8 | n/a | 2.5 | 4 |
| Tb927.10.2350 | pyruvate dehydrogenase complex | 2 | n/a | 2.3 | 1 |
| Tb927.10.4280 | Complex III cytochrome b/c | 4.1 | n/a | 19 | 2 |
| Tb927.5.3040 | Cytochrome c oxidase complex | 2.5 | n/a | 4.0 | 1 |
| Tb927.4.4990 | ubiquinol-cytochrome c reductase | 1.7 | n/a | 3.2 | 1 |
| Tb927.9.4310 | tricarboxylate carrier, putative | 2 | n/a | 2.2 | 1 |
| Regulation | |||||
| Tb927.11.15010 | NEK21 protein kinase | 2.5 | n/a | 3.1 | 4 |
| Tb927.2.4200 | protein kinase | 1.5 | 0.63 | 1.7 | 4 |
| Tb927.10.8050 | Protein phosphatase | 2.1 | n/a | 1.8 | 2 |
| Tb927.7.2680 | ZC3H22 | 1.9 | 0.57 | 1.8 | 7 |
| Other | |||||
| Tb927.8.7730 | Dihydroceramide synthase | 1.7 | n/a | 2.1 | 2 |
| Tb927.9.7470 | purine nucleoside transporter NT10 | 1.9 | n/a | 12 | 1 |
| Tb11.02.5400 | cystathionine beta-synthase | 2 | 0.58 | 2.3 | 1 |
| Tb927.7.1320 | Heat shock protein HSP10 | 2.1 | n/a | 2.2 | 1 |
| Tb927.10.7700 | ABC transporter, putative | 1.5 | n/a | 2.7 | 2 |
| Tb927.11.2410 | Flagellar membrane protein | 2.7 | n/a | 11 | 1 |
| Tb927.8.4050 | Flagellar membrane protein | 2 | n/a | 5.9 | 1 |
| Tb927.9.13200 | Unknown function | 4 | 0.46 | 15.8 | 2 |
| Tb927.9.13570 | Unknown function | 1.8 | 0.62 | 2.1 | 2 |
UAU6: number of UA(U)6 motifs in manually annotated 3'-UTR.
n/a = not significantly regulated.