Figure 1. Analysis of cell population in irradiation mix BM chimera mice generated with WT vs. Spp1^−/− donor cells.

WT (CD45.1/CD45.2) and Spp1^−/− mice (CD45.2) to irradiated WT recipients (CD45.1). (a) Flow cytometry to detect indicated cell populations of WT (labeled with blue) and Spp1^−/− (labeled with red) donor origins in the BM and spleens 7-wk after BM cell transfer. (b) Ratios (Spp1^−/−/WT) of donor cell numbers in the indicated organs. Mɸ: macrophages. Datasets are representative of two independent experiments. (c, d) Analyzing hematopoietic progenitors in the BM of the mixed BM-chimera mice at 3 weeks after BM transplantation. Representative flow plots (c) and frequencies of WT and Spp1^−/− cells in indicated progenitors (d). (e) Instead of total BM cells, LSK cells from WT (CD45.1) and Spp1^−/− (CD45.2) were transferred to WT recipients (CD45.1/CD45.2). Ratios of WT and Spp1^−/− progenitor cells were assessed by flow cytometry at 3 weeks after transfer. Each dataset is a representative of two independent repeats (5 mice per group)(c–e). (f) Equal numbers of BM cells from naive WT (CD45.2) and Spp1^−/− (CD45.1) mice were transferred to irradiated WT recipients (CD45.1/CD45.2). Six days after BM cell transfer, the ratio of WT and Spp1^−/− in Lin⁻c-kit⁺, LSK (Lin⁻Sca-1⁺c-kit⁺), and Lin⁻Sca-1⁺c-kit⁻ cells in the BM was examined. n=3 per each circle on day 6. Data were from three independent experiments. Error bars indicate ± SEM. * p<0.05 (Student’s t-Test).