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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Immunol. 2017 Jul 26;199(5):1635–1646. doi: 10.4049/jimmunol.1700560

Figure 3. Provision of sIgM rescues IgG plasma cell differentiation in μs−/− B cells.

Figure 3

(A) Bone marrow BM) chimeras were created by transfer of 50% WT BM (CD45.1) and 50% μs−/− BM (CD45.2) into lethally-irradiated C57BL/6 mice. Chimeras (n=4) were infected with A/PR8. (B) WT B and μs−/− B cells were identified by congenic markers CD45.1 and CD45.2, respectively. B cells originating from WT or μs−/− BM were stained for CD138+ expression. (C) Graphs show mean frequencies ± SD of IgM+CD138+ and IgM−CD138+ plasma cells of B cells derived from WT (CD45.1) and μs−/− (CD45.2) cells. (D) Shown are mean numbers ± SD of A/PR8-specific IgG and IgM ASC after WT (CD45.1) and μs−/− (CD45.2) B cells from medLNs of chimeras at day 8 after A/PR8 infection were FACS-sorted and analyzed by ELISPOT. Data are representative of two independent experiments. *p<0.05, **p<0.005, ***p<0.0005 by unpaired two-tailed Student’s t-test.