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. Author manuscript; available in PMC: 2018 Sep 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2017 Apr 14;331:24–32. doi: 10.1016/j.taap.2017.04.016

Fig. 8. Luteolin inhibits binding of Nrf2 to ARE sites on the Bcl-2, Bcl-XL, and HO-1 gene promoters in Cr(VI)-transformed cells using ChIP assay.

Fig. 8

Cr(VI)-transformed cells in the presence or absence of luteolin (20 μM) were fixed with formaldehyde and cross-linked. The chromatin was sheared and immunoprecipitated with anti-Nrf2 antibody or control IgG. Binding of Nrf2 to Bcl-2, Bcl-XL, and HO-1 promoters was analyzed by PCR using specific primers for ARE regions of their promoters (A, C, and E). ChIP and quantitative RT-PCR analysis was performed. The immunoprecipitated DNA was normalized to the input levels and plotted (B, D, and F). Data are mean ±SE (n=6). * indicates a p < 0.05, compared to levels obtained without luteolin. BEAS-2B-Cr; Cr(VI)-transformed BEAS-2B cells, Lut; luteolin.