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. Author manuscript; available in PMC: 2018 Aug 22.
Published in final edited form as: Circulation. 2017 Jun 1;136(8):729–742. doi: 10.1161/CIRCULATIONAHA.116.026240

Figure 1. Loss of Traf2 in the heart induces cardiomyocyte death, pathological remodeling and cardiac dysfunction.

Figure 1

A, Western blotting for Traf2 and a-tubulin (loading control) in cardiac extracts from 2 months old wild-type mice subjected to TAC or sham surgery for 2 weeks. B, Western blotting for Traf2 and a-tubulin in cardiac extracts from 2 months old wild-type mice subjected to MI or sham surgery for 2 weeks. C, Western blotting for Traf2 and a-tubulin in cardiac extracts from Traf2fl/fl and Traf2fl/fl-αMHC-Cre mice at 2 months of age. D, Masson's trichrome-stained, paraffin-embedded sections from the hearts of Traf2fl/fl and Traf2fl/fl-αMHC-Cre mice. Myocardial fibrosis was determined by MetaMorph software. *P < 0.01 versus Traf2fl/fl. N = 7 per group. Scale bars, top: 1 mm; bottom: 50 μm. E, TUNEL-positive nuclei of paraffin-embedded sections from the hearts of Traf2fl/fl and Traf2fl/fl-aCre mice. *P < 0.05 versus Traf2fl/fl. N = 6 per group. Scale bars, 10 μm. F, Western blotting for the indicated proteins from cardiac extracts of the indicated mice. G, Plasma HMGB1 levels from Traf2fl/fl and Traf2fl/fl-aCre mice. *P < 0.05 versus Traf2fl/fl. N = 5 per group. H and I, Echocardiographic assessment of fractional shortening (FS) and left ventricular dimension in diastole (LVED) in the indicated mice at 2 months of age. *P < 0.05 versus Wt, aCre, or Traf2fl/fl. J, Heart weight to body weight ratio (HW/BW) of the indicated mice. *P < 0.05 versus Wt, aCre, or Traf2fl/fl. Mann-Whitney U-test was used in D, E, G. Kruskal-Wallis test followed by post-hoc Mann-Whitney U-test with Bonferroni's correction was performed in H-J.